It was to intend to construct Pichia pastorisexpression system of neuritin,to obtain large amount of active Neuritin protein and study its neurobiological function.Encoding cDNA sequence of neuritin was amplified by PCR,then the fragment was digested and ligated into shuttle vector pPIC9K.The neuritin-pPIC9K recombinant was transformed into E.coliDH5α,and was confirmed by PCR and sequencing.The recombinant was linearized by restriction enzymeSalⅠ,and transformed into yeast GS115 by electric shock.After scre...
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