【摘要】 目的:以乳酸菌及盘基网柄菌为代表研究芸豆PHA对其增殖的影响。方法:采用纸片扩散法观察芸豆PHA对乳酸菌增殖的影响;采用液体振荡和贴壁培养观察芸豆PHA对盘基网柄菌生长增殖的影响。结果:1)乳酸菌培养12 h,1 mg/ml组滤纸片周围出现大的菌落,继续培养各浓度含芸豆PHA滤纸片周围均出现生长圈,对照组无生长圈;2)振荡培养24h时,低剂量各芸豆PHA组细胞密度比值最大,1mg/ml组达2.25,高于2.5mg/ml组细胞密度不随时间而增加,5.0和10 mg/ml组贴壁培养12h,盘基网柄菌不运动为死亡状态。结论:芸豆PHA对不同种类生物的生长增殖具有广泛的调节作用,其作用呈明显的剂量-效果关系。更多还原

【Abstract】 Objective:To study the effect of phytohemagglutinin(PHA) on lactobacillus and dictyostelium discoideum’s growth and reproduction.Method:The effects of PHA from Yunnan kidney bean on multiplication of lactobacillus has been observed and recorded by paper disk diffusion method;shaking culture system has been used to observe the effects of PHA from Yunnan kidney bean on multiplication of dictyostelium discoideum.Result:Experimental results showed that:1) after 12 hours of lactobacillus culture,big colonies appeared around filter paper in the group of concentrations of PHA at 1 mg/ml,and continued culture different concentration of PHA,growth rings all appeared around pieces of paper,but growth rings didn’t appear in control group.2) after 24 hours of shaking culture,the rate of cell density is the biggest in groups of Low concentrations PHA,the 1 mg/ml group reached 2.25,when concentrations of PHA were more than 2.5 mg/ml,cell density didn’t increase with the change of time,the 5.0 and 10.0 mg/ml group were cultivated by adherent culture for 12 hours,dictyostelium discoideum appeared no movement and acted as death.Conclusion:PHA plays a broad regulatory role on different kinds of creatures,the effects of the regulatory role shows a dose-effects depend manner.更多还原