【摘要】 目的:探讨内皮生长因子-C(vascular endothelial growth factor-C,VEGF-C)、黏附分子CD24在肺癌组织中的表达及其临床意义。方法:采用RT-PCR和免疫组化方法检测138例原发性肺癌患者肿瘤组织及癌旁组织中VEGF-C、CD24的表达水平。结果:肺癌组织中VEGF-C、CD24 mRNA及蛋白的表达均高于癌旁组织(P<0.05),两者在肺癌组织中的表达明显正相关(P<0.05);有淋巴结转移组中VEGF-C、CD24 mRNA及蛋白的阳性表达量均高于无淋巴结转移组(P<0.05);VEGF-C、CD24表达与淋巴结转移、肿瘤TNM分期等临床病理特征间有明显相关性(P<0.05);VEGF-C、CD24蛋白表达与ⅢA期患者的短期预后有关,两者的mRNA水平与无病生存时间呈负相关。结论:VEGF-C、CD24在肺癌组织中均异常表达,可作为肺癌诊断的辅助标志物。更多还原

【Abstract】 Objective: To study the expressions of vascular endothelial growth factor-C(VEGF-C) and CD24 in lung cancer and their clinical significance,and to discuss the relationship between their expressions as well.Methods: The methods of RT-PCR and immunohistochemical staining are adopted.The expression levels of VEGF-C,CD24 mRNAs and proteins in 138 primary lung cancer patients 7cancer tissues and paracancerous tissues are detected.Results: The expression levels of VEGF-C CD24 mRNA and proteins in lung cancertissues are all higherthanthosein paracaneeroustissues(P<0.05).Thereis evident positive eorrelativity between the expressions of VEGF-C and CD24 in lung cancer(P<0.05).The expression levels of VEGF-C,CD24 mRNA and proreins in themetastasis group are all higherthanthoseinthe non-metastasisgroup(P<0.05).Thereis evident correlative-ty between the expression of VEGF-C,CD24 and lymph node metastasis,TNM staging(P<0.05).The expressions of VEGF-C,CD24 mRNA and proteins are both related to the short-term prognosis of the Stage IlIApatients.Conclusion: VEGF-C and CD24 are both abnormally expressed in lung cancer tissues,which can be used as a marker of lung cancer diagnosis.更多还原