【摘要】 目的:研究重组人干扰素α1b对呼吸道合胞病毒感染小鼠外周血T淋巴细胞亚群及肺组织病理学的影响。方法:将40只BALB/c小鼠随机分为5组,分别为正常对照组、感染模型组、重组人干扰素α1b 3.125μg剂量组、重组人干扰素α1b 12.5μg剂量组和重组人干扰素α1b 50μg剂量组。建立感染小鼠模型成功后,连续雾化干预5天,第6天摘眼球取血、肝素化处理,流式细胞术检测小鼠外周血CD3+CD4+、CD3+CD8+水平。剖取小鼠左肺立即固定于10%中性福尔马林溶液24小时,石蜡包埋、切片行HE染色,光镜下观察小鼠肺组织病理学改变。结果:与正常对照组比较,其余各组小鼠外周血CD3+CD8+水平均显著降低,CD3+CD4+与CD3+CD4+/CD3+CD8+水平均显著升高,除重组人干扰素α1b 50μg剂量组CD3+CD4+水平外,其余各组差异均有统计学意义(P<0.05);与感染模型组比较,其余各组小鼠外周血CD3+CD8+水平均显著升高,CD3+CD4+与CD3+CD4+/CD3+CD8+水平均显著降低,除重组人干扰素α1b 3.125μg剂量组CD3+CD8+水平外,其余各组差异均有统计学意义(P<0.05);重组人干扰素α1b 3.125μg、12.5μg、50μg剂量组间比较可见,随着药物剂量的成倍增加,CD3+CD8+水平呈递增趋势,CD3+CD4+与CD3+CD4+/CD3+CD8+水平呈递减趋势,除重组人干扰素α1b 3.125μg与12.5μg剂量组间P>0.05,其余各组间差异均有统计学意义(P<0.05)。肺组织病理学观察到感染模型组小鼠肺组织炎性损伤显著,雾化吸入重组人干扰素α1b后,肺组织炎性损伤程度显著降低。结论:重组人干扰素α1b对RSV感染小鼠外周血T淋巴细胞亚群及肺组织病理学影响显著,具有良好的抗病毒及免疫调节作用,并在一定范围内存在剂量-效应关系。更多还原

【Abstract】 Objective: To study the effect of recombinant human interferon α1b on T-lymphocyte subsets in peripheral blood and lung tissue pathology in mice infected by respiratory syncytial virus.Methods: 40 BALB/c mice were randomly divided into 5 groups,which were normalcontrol group,infection model group,recombinant human interferonα1b dose of 3.125μg group,recombinant human interferon α1b dose of 12.5 μg group and recombinant human interferon α1b dose of 50 μg group.After the success of establishing the infection model,continuous atomized intervention for 5 days,the peripheral blood was sampled from mice’s eyes and detected CD3+CD4+ and CD3+CD8+levels after heparinization on flow cytometer in the 6th day.Left lung tissue was immediately preserved in 10 % formalin solution for 24 hours,subsequently paraffin embedded,and sections were stained with hematoxylin and eosin;Observing lung tissue pathology change by optical microscope.Results: Compared with the normal control group,CD3+CD8+levels in peripheral blood of the rest groups were significantly reduced;CD3+CD4+ and CD3+CD4+/CD3+CD8+ levels were significantly increased;in addition to recombinant human interferon α1b dose of 50 μg group of CD3+CD4+ levels outside,differences between the rest groups were statistically significant(P<0.05).Compared with the infection model group,CD3+CD8+levels in peripheral blood of the rest groups were significantly increased;CD3+CD4+ and CD3+CD4+/CD3+CD8+ levels were significantly reduced;in addition to recombinant human interferon α 1b dose of 3.125 μ g group of CD3 + CD8 + levels outside,differences between the rest groups were statistically significant(P<0.05).Recombinant human interferon α 1b dose of 3.125 μ g,12.5 μ g and 50 μ g groups were compared between visibly,along with the doses were multiplying,CD3 + CD8 + levels presented increasing tendency,CD3 + CD4 + and CD3 + CD4 + /CD3 + CD8 + levels presented decreasing tendency;in addition to recombinant human interferon α 1b dose of 3.125 μ g and 12.5 μ g groups P>0.05,the difference between groups were statistically significant(P<0.05).Lung tissue pathology showed severe inflammatory damage significantly.After atomized inhalation of recombinant human interferon α 1b,inflammatory damage degree of lung tissue significantly reduced.Conclusions: Recombinant human interferon α 1b has distinct impact on the T-lymphocyte subsets in peripheral blood and lung tissue pathology in mice infected by respiratory syncytial virus,having obvious antiviral and immune adjustment effect,and within the scope of certain existing dose-effect relationship.更多还原