【摘要】 根据细菌群体感应信号降解酶(AiiA)基因设计简并引物,从海洋微生物ZD02基因组中扩增编码AiiA的基因aiiA,筛选其阳性克隆,测序后分析其基因序列。结果表明:筛选到的阳性克隆ZD02-aiiA序列全长753bp(Genbank登录号:KC756214),存在一个开放阅读框(ORF),编码由250个氨基酸残基组成的多肽,预测分子量为28.1kDa,蛋白等电点为4.78。由该序列推导得到的氨基酸残基序列含有一个保守结构域(Lactamase-B)(34AA～235AA),并预测了AiiA的三级结构。以上研究为该序列的重组表达及其相关活性研究奠定了基础。更多还原

【Abstract】 By using a pair of degenerate primers designed according to the known conserved sequence of aiiA,the gene aiiA encoding the quorum sensing signal degrading enzyme ( AiiA) in the genome of marine bacterium ZD02 was amplified by PCR,and the PCR products ( ZD02 aiiA) were cloned,sequenced,and analyzed. The ZD02 aiiA was 753 bp ( Genbank accession number: KC756214) ,containing an open reading frame ( ORF) which encoded a polypeptide chain AiiA of 250 amino acids residues with a molecular weight of 28. 1 kDa and an isoelectric point value of 4. 78. A conservative domain ( Lactamase-B) ( 34AA-235AA) in the sequence was identified,and the 3D structure of deduced AiiA protein was further predicted. This study would provide a foundation for the expression of the sequence and its bio-activity analysis.更多还原