【摘要】 确定乙醇-硫酸铵双水相体系提取葡萄皮中总多酚为较优方法,在单因素试验基础上用响应面法优化提取条件。结果表明:乙醇-硫酸铵-水双水相体系在静态水浴条件下对总多酚的提取效果要高于超声辅助和单独的乙醇提取。其最佳提取条件为:醇水比为6:4(v:v)、硫酸铵添加量0.1 g/mL、液料比8:1(mL:g)、抗坏血酸添加量0.7 g/L、水浴时间2 h、温度50℃,葡萄皮多酚提取量可达12.36 mg/g,与预测值拟合程度高。相同条件下浸提2次,提取率可达94%。更多还原

【Abstract】 Two phase extraction method was presented for extracting total phenols from grape skins.Based on single factor experiments,the optimal extraction was explored by response surface methodology.Results showed that the extraction yield obtained by static constant temperature water was higher than that obtained by ultrasonic-assisted and ethanol extraction.The optimal extraction conditions were obtained as follows: ethanol-water ratio of 6:4(v/v) and ammonium sulfate concentration 0.1 g/mL,liquid-material ratio of 8:1(mL/g),ascorbic acid concentration 0.7 g/L,extraction time of 2 h,extraction temperature of 50 ℃.Under these optimal conditions,the extraction content of phenol is 12.36 mg/g,which was very close to predicted value.Under the same condition extract twice,extraction rate can reach 94%.更多还原