【摘要】 目的:分离纯化由Yg菌株生产的壳聚糖酶并对其性质进行研究。方法:分段醇析法分离纯化壳聚糖酶;不连续SDS-PAGE电泳法测定其相对分子质量;对其最适反应温度、pH值以及其热稳定性进行研究。结果:壳聚糖酶相对分子质量为41.7 ku,最适反应温度为45℃,最佳反应pH为5.0~5.5。该酶4℃保存稳定性较好,65℃以上短时间内可以导致酶活性丧失。结论:分离纯化得到壳聚糖酶并对其性质进行了初步研究,为壳聚糖酶制剂的生产提供了理论依据。更多还原

【Abstract】 Objective: To purify the chitosanase produced by Yg strain,and research its property. Method:Purified chitosanase though stage ethanol extraction. Detected its relative molecular mass,the optimal reaction temperature and pH. Research the thermal stability of chitosanase. Result: As a result,the molecular weight of chitosanase was 41.7 ku. The optimal reaction temperature was 45 ℃ and pH value was 5.0~5.5. On 4 ℃ condition,chitosanase exhibited well stability,but it lost activity rapidly on condition over 65 ℃. Conclusion: The chitosanase was purified and its property was studied. The results provided a theoretical basis for the production of chitosanase.更多还原