【摘要】 建立一种快速测定百香果中绿原酸含量的方法。百香果果肉用微波辅助提取后,采用高效液相色谱法(HPLC)测定。色谱条件为:Eclipse XDB-C18色谱柱(4.6 mm×150 mm,5μm);流动相:A相为水+1%乙酸,B相为乙腈,采用梯度洗脱,最佳流速0.8 mL/min,检测波长320 nm,柱温40℃,进样量10μL。结果表明,绿原酸线性范围为19.80 mg/L-99.60 mg/L(R2=0.999 7),平均加标回收率为97.68%(n=5),绿原酸平均含量为20.53 mg/g。该方法快速、准确,溶剂用量少,为快速测定百香果中的绿原酸提供参考。更多还原

【Abstract】 A rapid method for the determination of chlorogenic acid in Passiflora eduis using high performance liquid chromatography(HPLC) method was established.The samples were extracted with method by microwave-assisted extraction and detected directly by HPLC method.The separation of chlorogenic acid was performed on Eclipse XDB-C 18 column(4.6 mm×150 mm,5 μm),using acetonitrile-water as mobile phase with gradient elution,at the flow rate of 0.8 mL/min.The detection was carried out at 40 ℃ with wavelength of 320 nm and injection volume of 10 μL.Under the optimized conditions,the calibration curve of chlorogenic acid was linearly over the ranges of 19.80 mg/L-99.60 mg/L(R 2 =0.999 7).The average content of chlorogenic acid in Passiflora eduis samples was found to be 20.53 mg/g and an average recovery rate of 97.68 %(n=5) was obtained.The proposed method has many advantages such as high sensitivity,fastness,as well as small amounts of organic solvent consuming.更多还原