【摘要】 建立高效液相色谱法同时分离测定无糖食品中多种糖和糖醇的含量。样品提取净化后,采用高效糖分析色谱柱分离(250 mm×4.6 mm;5μm)、乙腈/水梯度洗脱,流速:1.4 mL/min;柱温:35℃;蒸发光散射检测器检测,漂移管温度80℃,喷雾器温度34.8℃,进样量20μL。结果表明:木糖、木糖醇、果糖、山梨醇、葡萄糖、蔗糖、麦芽糖醇、乳糖、乳糖醇进样量1 mg/mL~10 mg/mL的范围线性良好,相关系数为0.994 6~0.999 3;RSD<2%,回收率平均在89.2%~110.9%之间。本方法该方法灵敏度高,重复性好,可同时对无糖食品中的4种糖醇和5种糖进行分离与检测。更多还原

【Abstract】 A quantitive assay for the determination of sugar and sugar alcohol in the sugar free foods was established by high performance liquid chromatography(HPLC).Sample was extracted and the separation was performed with high performance carbohydrate column(250 mm × 4.6 mm,5 μm),acetonitrile /water as mobile phase.Flow rate:1.4 mL/min;column temperature:35 ℃;Evaporative Light Scattering detector conditions: drift tube temperature: 80 ℃,atomizer temperature: 34.8 ℃,sample volume 20 μL.The result showed that the xylose,xylitol,fructose,sorbitol,glucose,sucrose,maltitol,lactose,lactitol showed distinct linearity relation ship with the concentration in the range of 1mg/mL-10 mg/mL,the correlation coefficient was 0.994 6-0.999 3,RSD was <2 %,the recovery was 89.2 %-110.9 %.The method was accuracy,sensitivity and feasibility to separate and determinate the sugar and sugar alcohol in sugar free foods.更多还原