Pseudomonas stutzeri F4 with high degradation activity of AFB1 was isolated and identified previously.In this study,the fermentation conditions for AFB1-degrading enzyme(ADE) production by this strain were optimized.The effects of inoculum age,inoculum size,initial pH,fermentation time and temperature on ADE activity were tested by single factor tests.Based on the results of single factor tests,the fermentation conditions were then optimized using response surface methodology.When inoculum size and initial ...