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热处理对黄花梨冷藏品质和活性氧代谢的影响
Effect of Hot Air Treatment on the Quality and Reactive Oxygen Species Metabolism of Cold Stored Huanghua Pear Fruits
【摘要】 为探讨热处理对黄花梨冷藏品质及其氧化还原水平的作用,黄花梨果实采后先经40℃热空气处理12h,再在1℃贮藏120d,随后20℃存放7d,监测果实硬度、可溶性固形物、电导率、丙二醛(MDA)含量、超氧阴离子自由基(O2-·)生成速率、可溶性蛋白含量和活性氧清除酶活性的变化。结果显示:热处理可显著抑制果实可溶性蛋白分解,提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性,降低O2-·生成速率和MDA含量,同时可以保持果实硬度和可溶性固形物,延缓电导率上升。研究表明,贮前热空气处理(40℃,12h)可延缓黄花梨果实的衰老变质,其作用与较高的抗氧化水平有关。
【Abstract】 To explore the effect of thermal treatment on the quality and reactive oxygen species(ROS) metabolism of cold stored Huanghua pear,postharvest fruit samples were treated in hot air at 40℃for 12 h before storage at 1℃for 120 d followed by at 20℃for another 7 days,and changes in fruit firmness,conductivity,MDA content and superoxide anion radical generation rate,soluble protein content and ROS scavenging enzyme activities during the whole storage period were monitored.Results showed that fruit firmness,soluble protein content,and the activities of SOD,CAT and POD in hot air treated fruits were higher than control fruits,whereas opposite results were observed for soluble solid content,electrolyte leakage,MDA content and superoxide anion radical production rate.These results indicate that hot air treatment can improve the quality of Huanghua pear fruits during storage,and the mechanism might be related to the activation of antioxidant enzymes and the reduction of ROS accumulation.
【Key words】 Huanghua pear; thermal treatment; cold storage; senescence; antioxidant activity;
- 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2013年02期
- 【分类号】TS255.4
- 【被引频次】31
- 【下载频次】250