Objective:To create and optimize the purification craft of polyphenols from Capsicum annuum leaves. Methods:Macroporous resin was chosen under the condition of static adsorption and desorption,then using single-factor approach to study the optimal column chromatography conditions. Result:HPD-100 resin has better adsorption and desorption properties than that of other resins. The optimal column chromatography conditions:resin column diameter to height ratio 1:4,sample polyphenol concentration between 0. 6 ~ ...