Lactobacillus helveticus strain AJT,isolated from traditional fermented dairy samples in Inner Mongolia,China,its antibacterial substance’s production increased continuously from 6h,and reached a maximum after 20h. Obtained through ethyl acetate and methanol extraction,the antibacterial substance,had strong antibacterial activity against Gram - positive bacteria such as Staphylococcus aureus,Gram - negative bacteria such as Escherichia coli and fungi such as Penicillium expansum after eliminating the effect...