【摘要】 海藻糖作为一种功能糖,在生命科学、医药、农业、食品工业、化妆品工业等领域都有着广阔的应用前景。长期以来,由于海藻糖制取和提取上存在的困难使海藻糖的价格昂贵。试验通过基因工程技术获得一株具高效表达海藻糖合酶的大肠杆菌,该菌海藻糖合酶产量稳定,产酶酶活高。该大肠杆菌基因工程菌产海藻糖合酶最适温度为50℃,pH 7.0~7.5,底物质量浓度为0.3 g·mL-1,反应时间为8 h,其最高转化率62.14%,每克大肠杆菌干菌体产酶酶活达到311.42 U/g。更多还原

【Abstract】 As a functional sugar,trehalose has a broad application prospects in life sciences,medicine,agriculture,food industry,cosmetics industry and other fields.Because of the difficulties on trehalose production and extraction,the price of trehalose is high.By genetic engineering technology,obtain a recombinant E.coli with high enzyme activity.Through verification testing,the recombinant E.coli has a higher activity,then research its enzymatic conversion conditions for industrial production.The optimum reaction condition of trehalose synthase is pH 7.0~7.5,the substrate con centration of 0.3 g· mL-1,the temperature of 50 ℃,the substrate concentration of 30% and the reaction time of 8 h.The highest conversion rate is 62.14%.Trehalose synthase activity of recombinant E.coli reaches 311.42 U/g.更多还原