【摘要】 以柑橘籽为原料,研究柑橘籽类柠檬苦素亚临界水萃取提取工艺及体外抗肿瘤作用。采用亚临界水提取柑橘籽类柠檬苦素,利用响应面分析法优化其提取工艺,并对柑橘籽类柠檬苦素对肿瘤细胞株抑制作用进行分析。结果表明柑橘籽类柠檬苦素萃取最佳工艺条件为萃取时间为18.8 min,萃取温度为188.3℃,物料粒径为71.3目(210μm),液料比值为23.3(mL/g)。而且类柠檬苦素对肿瘤细胞株有较强的抑制作用。更多还原

【Abstract】 The extraction technology and anti-tumor of limonoids from citrus seed were discussed.Limonoids from citrus seed was extracted by subcritical water,the optimum extraction conditions of limonoids from citrus seed were determined by response surface methodology,inbibitional effects of limonoids to cancer cell lines were analyzed.The optimum extraction conditions were as follows: extraction time was 18.8 min,the extraction temperature was 188.3 ℃,the particle size of the material was 71.3 meshes(210 μm),the ratio of solution and material was 23.3(mL/g),and limonoids from citrus seed had strong inhibition effects on cancer cell lines.更多还原