【摘要】 目的:探讨HiLo训练对大鼠骨骼肌PK及其转录调节因子HIF-1α和ChREBP的影响。方法:40只适应训练后8w龄SD大鼠随机分为低住安静组(LC)、低住低练组(LoLo)、高住安静组(HC)、高住低练组(HiLo)。高住组每天低氧暴露12 h(氧浓度13.6%,相当于海拔3500 m),低练组进行35 m/min、1 h/d、5d/w跑台训练、共训练4w。比色法检测腓肠肌PK活性,RQ-PCR和WB检测PK、HIF-1α和ChREBP的基因和蛋白表达。结果:(1)低氧显著降低PK活性(P<0.01),运动显著升高PK蛋白表达(P<0.05),低氧和运动均能显著提高PK mRNA表达(P<0.01)且具有显著的交互作用(P<0.01);(2)低氧和运动均能显著提高HIF-1αmRNA和蛋白表达(P<0.01)且具有显著的交互作用(P<0.01);(3)运动对ChREBP mRNA和蛋白表达影响显著(P<0.05和P<0.01),且低氧和运动具有显著的交互作用(P<0.01)。结论:4周HiLo训练能够降低大鼠腓肠肌安静状态下PK活性,并通过上调其转录调节因子HIF-1α和ChREBP基因和蛋白的表达诱导PK基因表达,有利于PK在机体安静状态储备能量和在运动状态激发活性,是HiLo训练适应的结果。更多还原

【Abstract】 Purposes: To evaluate the effects of HiLo training on pyruvic ketolase and the expression of HIF-1α and ChREBP in rat skeletal muscles.Methods: After adaptive training,40 8-week-old male SD rats are divided into living-low quiet control group(LC),living-low training-low group(LoLo),living-high quiet control group(HC) and living-high training-low group(HiLo).All living-high groups stay in the environment with 13.6% oxygen concentration,about the altitude of 3500 m,for 12h/day.All training groups undergo treadmill training with 35m/min for 1hour/day,5days/ week,4 weeks.Gastrocnemius is obtained after 24hs of last training.The activity of PK is detected by colorimetry.Technology of Real Time Quantitative PCR and Western blotting are adopted in order to test the genes expression and protein level changes of PK,HIF-1α and ChREBP.Results:(1) Hypoxia reduces PK activity significantly(p<0.01).Training increases PK protein level significantly(p<0.05).Hypoxia and training increase PK mRNA level significantly(p<0.01) and there is significantly interaction.(2) Hypoxia and training increase HIF-1α mRNA and protein level significantly(p<0.01) and there is significant interaction.(3) Training increases ChREBP mRNA and protein level significantly(p<0.05 or p<0.01),and there is significantly interaction between hypoxia and training(p<0.01).Conclusions: 4 weeks of HiLo training can reduce resting rat’s gastrocnemius activity of PK,and the up-regulation of gastrocnemius HIF-1α and ChREBP gene and protein expression can induce PK gene expression,which is beneficial to PK for the body to store energy in resting state and stimulating activity at state of motion,which is the result of HiLo training accommodation.更多还原