【摘要】 目的探讨褪黑素对锰致小鼠运动障碍及纹状体损伤的影响。方法小鼠84只,均分为6组,分别为对照组及低、中、高Mn组,褪黑素(MT)对照组和MT+高Mn组。第5、6组提前皮下注射给予5 mg/kg MT,2 h后,第2~4和6组分别腹腔注射给予12.5、25、50和50 mg/kg MnCl2,连续2周。观察小鼠的自主活动情况,检测纹状体GSH-Px、SOD和γ-GCS的活力、表达及蛋白水平。结果随着锰浓度的升高,自主活动和站立次数逐渐减少;纹状体γ-GCS、GSH-Px和SOD活力、蛋白表达水平逐渐下降,并呈剂量-效应关系。与高锰组比较,MT干预组自主运动和站立次数增加明显(P<0.05,P<0.01);γ-GCS、GSH-Px和SOD活力、阳性表达及蛋白水平均显著升高。结论本实验结果提示过量锰暴露可导致运动障碍及纹状体氧化损伤,褪黑素可能通过激活抗氧化酶GSH-Px、SOD和γ-GCS发挥对它的拮抗作用。更多还原

【Abstract】 Objective Study the effect of melatonin on manganese( Mn)-induced dyskinesia and striatum injury of mice. Methods Eighty-four mice were randomly divided into six groups: they were the control group,low-dose Mn group,mediumdose Mn group,high-dose Mn group,MT control group,and MT + high Mn group. The mice in fifth and sixth groups were injected s. c with 5 mg / kg MT in advance,two hours later,all the mice except control and MT groups were injected i. p with 12. 5,25,50,and 50 mg / kg MnCl 2,respectively everyday for two weeks. Observe the activity status of mice and detect the activity and expression levels of GSH-Px,SOD and γ-GCS in striatum. Results With the elevation of Mn exposure level,the frequency of activity and stand reduced,while the activity and expression of γ-GCS,GSH-Px and SOD in striatum were rised in a dose-dependent manner. Comparing with high-dose Mn group,autonomic activity and stand of mice in MT intervention group were increased significantly( P < 0. 05,P < 0. 01,respectively),the activities and expression level of γ-GCS,GSH-Px,and SOD were also elevated obviously. Conclusion The results suggested that the excessive exposure of Mn might induce dyskinesia and oxidative damage of striatum,while melatonin could antagonize the effect by activating the antioxidant enzymes such as GSHPx,SOD,and γ-GCS.更多还原