【摘要】 目的 观察碱性成纤维细胞生长因子(bFGF)在多孔高密度聚乙烯(Medpor)-软骨复合支架血管化中的应用效果,探讨其使用意义。方法 构建兔耳软骨与Medpor复合支架模型,植于兔背部皮下,将实验动物分成2组:耳软骨与Medpor材料直接连接组(A组);耳软骨与Medpor材料直接连接后,bFGF处理组(术中及术后2 d,每日局部注射bFGF 100 ng,B组);置入后第3、7、14、28天后取出复合支架,行大体和组织学观察,免疫组织化学法(SABC法),检测Medpor片边缘及内部CD34表达,并行微血管密度(MVD)计数。结果 B组术后早期Medpor材料周边纤维组织增生及血管化较A组明显,但后期血管化程度与A组无明显差异。结论 术后早期应用bFGF对复合支架的纤维血管化过程中起到明显的促进作用,加强Medpor与软骨片的连接,增强复合支架的稳定性及抗感染力。更多还原

【Abstract】 Objective To observe the application effect of basic fibroblast growth factor(bFGF) in the medpor-cartilage scaffold vascularization,and explore its significance.Methods Build rabbit ear cartilage with Medpor bracket model,implanted in rabbits subcutaneously,and the animals were divided into two groups:the ear cartilage and Medpor material directly connected group(Group A);the ear cartilage directly connected with medpor,and the scaffold was pretreated with bFGF(100 ng/d ×3 d,Group B).At the 3,7, 14,28 days after implantation,remove the composite scaffold for gross and histological observation,immunohistochemical method(SABC method) detection of CD34 expression in composite scaffolds and parallel microvessel density(MVD) count.Results Proliferation of fibrous tissue and vascularization in the early postoperative Group B than Group A was significantly,but no significant difference in the late vascularization with group A.Conclusion Early postoperative application of bFGF play a significant role in promoting for composite scaffolds fibrovascular process,and strengthen the connection between the Medpor material and cartilage piece,enhance the stability of the composite scaffold and anti-infectious.更多还原