【摘要】 目的建立紫苏叶药材中挥发油类成分指纹图谱及含量测定方法。方法选用(5%-苯基)-甲基聚硅氧烷毛细管色谱柱(30 m×0.32 mm,0.25μm),以程序升温的方法进行指纹图谱研究,同时采用GC-MS的方法进行成分鉴定;通过相似度分析、主成分分析、聚类分析等3种方法对不同产地的紫苏叶药材中挥发油类成分进行分析研究,比较不同产地的差异。结果建立了紫苏叶药材中挥发油类成分的指纹图谱和含量测定方法。通过计算机谱库检索,鉴定出20个化学成分,其中主要成分为2-己酰呋喃、紫苏醛、β-石竹烯、芹菜脑。采用相似度分析、主成分分析、聚类分析3种方法对紫苏叶进行指纹图谱分析,得到了较为一致的结论。结论通过对紫苏叶药材系统的质量研究,建立了药材的指纹图谱及含量测定方法,并反映了紫苏叶在地域方面存在差异,为药材的选择提供了一定依据。更多还原

【Abstract】 Objective To establish the fingerprint of the volatile oil in Folium Perillae and its determination method.Methods The fingerprint analysis was performed by with temperature programmed method.5%-Phenyl Methyl Siloxane Column(HP-5,30 m × 0.32 mm,0.25 μm) was used.Identification of constituents was carried out by Gas Chromatography-Mass Spectrometry methods.The variations brought by origin differences of volatile oil were investigated by similarity analysis,principal component analysis and cluster analysis.Results A total of 20 constituents were identified in Folium Perilla which the main components were 2-Acety furan,Perilla aldehyde,β-Caryophyllene and Apiole.Conclusion The systematic research of the Folium Perilla can be used to choose the high quality medicinal materials.更多还原