【摘要】 目的探讨过氧化物酶体增殖物激活受体-γ(PPAR-γ)在自发性高血压大鼠(SHR)胸主动脉的表达和瑞舒伐他汀(ROS)的干预作用。方法 16只6周龄健康雄性SHR随机分入SHR组和ROS组,每组8只;另选8只同龄雄性Wistar-Kyoto大鼠(WKY)作为对照(WKY组),喂养8周后抽取血液,处死,获取胸主动脉段,行苏木精-伊红染色并应用计算机图像分析系统测量中膜厚度/管腔内径(MT/LD)、中膜面积/管腔面积(MA/LA)比值,以双抗体夹心生物素-亲和素酶联免疫吸附试验测定法检测血清白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α、高敏C反应蛋白(hs-CRP)水平,取主动脉组织采用实时定量聚合酶链反应和Western印迹法测定PPAR-γ基因和蛋白表达水平。结果 SHR组10、14周龄的收缩压均显著高于同组6周龄(P值均<0.01),而WKY组、ROS组不同周龄间收缩压的差异均无统计学意义(P值均>0.05)。SHR组6、10、14周龄的收缩压均显著高于WKY组同周龄(P值均<0.01),而ROS组10、14周龄的收缩压均显著低于SHR组同周龄(P值均<0.01)。SHR组的血清IL-6、TNF-α、hs-CRP水平,以及MT/LD、MA/LA比值均显著高于WKY组和ROS组(P值分别<0.05、0.01),ROS组与WKY组间差异均无统计学意义(P值均>0.05)。SHR组PPAR-γmRNA、蛋白表达水平显著高于WKY组(P值均<0.01),但显著低于ROS组(P值均<0.01)。结论PPAR-γ可能与高血压血管重构有关,ROS可抑制炎症,促进血管中的PPAR-γ表达,改善血管重塑。更多还原

【Abstract】 Objective To investigate the expression of peroxisome proliferator-activated receptor gamma(PPAR-γ)and the effect of rosuvastatin on thoracic aorta remodeling in spontaneous hypertensive rat(SHR). Methods Sixteen SHRs were randomly divided into SHR group (n=8) and rosuvastatin(ROS)group (n=8). Eight age-matched male Wistar-Kyoto(WKY)rats were taken as controls.The animals were sacrificed after eight- week treatment.The ratios of media thickness to lumen diameter(MT/LD)and media area to lumen area(MA/ LA)of thoracic aorta were measured by computer image analysis.Serum levels of interleukin-6(IL-6),tumor necrosis factor(TNF)-α,all high sensitivity C-reactive protein(hs-CRP)were detected by ABC-enzyme-linked immunosorbent assay(ELISA).The mRNA and protein expression of thoracic aortic PPAR-γwere detected with real-time-polymerase chain reaction and Western blot,respectively.Results Systolic pressure in 10-and 14- week SHRs were significantly higher than that in 6-week SHRs(both P<0.01).But there was no significant difference in systolic pressure between rats of different ages in both WKY group and ROS group(all P>0.05). Systolic pressure in SHR group were significantly higher than thos in WKY group at the age of 6,10 and 14 weeks (all P<0.01),while systolic pressure in ROS group were significantly lower than that in SHR group at the age of 10 and 14weeks(all P<0.01).Serum levels of IL-6,TNF-αand hs-CRP in SHR group were significantly higherthan those in WKY group and ROS group(all P<0.05),so were MT/LD and MA/LA(all P<0.01).Compared with SHR group,the expression of PPAR-γmRNA and protein of thoracic aorta were significantly increased in ROS group and decreased in WKY group(all P<0.01).Conclusion PPAR-γis likely to be related to vascular remodeling.Rosuvastatin can increase the expression of PPAR-γand improve vascular remodeling so as to inhibit inflammatory reaction in SHRs.更多还原