【摘要】 目的检测肿瘤坏死因子样弱凋亡诱导因子(tumor necrosis factor-like weak inducer of apoptosis,TWEAK)及其受体成纤维细胞生长因子诱导因子14(fibroblast growth factor inducible 14,Fn14)mRNA及蛋白在大鼠骨关节炎(osteoarthritis,OA)关节软骨和滑膜中的表达情况,初步了解TWEAK及其受体Fn14在OA发病中的作用。方法 42只大鼠中随机选取18只行膝关节前交叉韧带切断(anterior cruciate ligamenttransection,ACLT)制作OA模型作为实验组,另外18只接受手术但不切断前交叉韧带作为假手术组,剩余6只作为正常对照组。饲养至1、2、4周时,实验组及假手术组随机各取6只处死,6只正常对照组在第4周处死,采集所有动物关节软骨及滑膜组织,用实时定量PCR及Western blot分别检测TWEAK和Fn14在mRNA及蛋白水平的表达情况。结果术后第2和第4周实验组动物关节滑膜中TWEAK及Fn14的mRNA及蛋白表达水平显著上调,与假手术组及正常对照组比较,差异均有统计学意义(P<0.05);术后第2周实验组动物关节软骨中TWEAK及Fn14的mRNA及蛋白表水平与假手术组及正常对照组比较,差异均有统计学意义(P<0.05)。结论 TWEAK及其受体Fn14的mRNA及蛋白表达水平在大鼠OA关节软骨及滑膜组织中的表达显著增高,它们可能是参与OA的重要细胞诱导因子。更多还原

【Abstract】 Objective To detect the expressions of tumor necrosis factor-like weak inducer of apoptosis(TWEAK) and its receptor fibroblast growth factor inducible 14(Fn14) mRNA and protein in the articular cartilage and synovium of rat osteoarthritis(OA) model,and to understand the preliminary role of TWEAK and its receptor Fn14 in osteoarthritis.Methods In the 42 rats,18 were randomly selected to establish an OA modle by the operation of anterior cruciate ligament transection(ACLT) as the experimental group,18 received the surgery without ACLT as the sham group,and the other 6 were treated as the normal control group.At the timepoint of the 1st,2nd and 4th week,one third of rats in every group were sacrificed and used for collecting articular cartilage and synovium tissues.The levels of TWEAK and Fn14 mRNA and protein were detected by the methods of real-time PCR and Western blot,respectively.Results TWEAK and its receptor Fn14 mRNA and protein expressive levels in synoyium of the model group at 2 and 4 weeks after operation were significantly increased,compared with the respective sham group and normal control group(P<0.05);while TWEAK and its receptor Fn14 mRNA and protein expression only in the model group cartilage 2 weeks significantly increased compared with the respective sham group and normal control group(P<0.05).Conclusions The mRNA and protein levels of TWEAK and its receptor Fn14 in articular cartilage and synovium tissue of rats with OA are significantly higher than those in the normal ones,which may be the important cell-inducible factors in the process of(OA) pathogenesis.更多还原