【摘要】 采用制备型高速逆流色谱对鬼臼中鬼臼毒素类成分进行分离纯化。实验经优化获得的分离条件为:先用正己烷-乙酸乙酯-甲醇-水(2∶3.5∶2∶3.5,V/V)上相为固定相,下相为流动相洗脱48分钟后,换用正己烷-乙酸乙酯-甲醇-水(4∶5∶4∶5,V/V)下相进行洗脱;转速为1200r/min;流速为50mL/min;进样体积50mL;进样浓度20mg/mL;共收集到六种高纯度化合物,经鉴定为鬼臼毒素、山荷叶素、4’-去甲基鬼臼毒素、异苦鬼臼毒酮、4’-去甲基去氢鬼臼毒素和去氢鬼臼毒素。更多还原

【Abstract】 Preparative separation of podophyllotoxin lignans in Dysosma versipellis(Hance)M.Cheng was conducted by high speed counter-current chromatography(HSCCC)with a pair of two solvent systems consisting of n-hexane-ethyl acetate-methanol-water(2∶3.5∶2∶3.5,V/V)and(4∶5∶4∶5,V/V).The optimized separation conditions were as follows:the upper phase of n-hexane-ethyl acetate-methanolwater(2∶3.5∶2∶3.5,V/V)was used as stationary phase,the lower phase was used as mobile phase at the first 48min,then the lower phase of n-hexane-ethyl acetate-methanol-water(4∶5∶4∶5,V/V)was used as mobile phase;rotation speed is 1200rpm;the flow rate is 50mL/min;sample volume is 50mL;sample concentration is 20mg/mL.Six compounds with high purity were collected and identified as podophyllotoxin,diphyllin,4′-demethylpodophyllotoxin,isopicropodophyllone,dehydropodophyllotoxin,4′demethyldehydropodophyllotoxin.更多还原