【摘要】 目的 比较裸鼹鼠与小鼠的自噬调节，以期为裸鼹鼠高自噬、抗癌、衰老缓慢等生物学特性的研究提供参考。方法 利用电镜技术和蛋白印迹检测新生裸鼹鼠和C57BL/6小鼠肝脏和肺脏组织的自噬水平。自噬干扰裸鼹鼠成纤维细胞和小鼠成纤维细胞，24、48、72 h CCK-8细胞计数法检测细胞的增殖情况，蛋白印迹检测各组细胞各个时间点Beclin 1的表达水平。结果 与C57BL/6小鼠比较，裸鼹鼠组织的LC3B表达显著较高。裸鼹鼠细胞对自噬抑制剂3-MA具有一定的耐药性,自噬诱导剂雷帕霉素对裸鼹鼠细胞的增殖抑制显著高于小鼠细胞。结论 裸鼹鼠组织具有高自噬水平,应对外界的自噬抑制压力时,裸鼹鼠细胞的自身调节能力明显高于C57BL/6小鼠。更多还原

【Abstract】 Objective A comparative study between naked mole rat and C57BL/6 mouse on autophagy regulation is conducted, providing a reference for the research of mechanism in high autophagy,anti-cancer, slow aging of NMR. Methods Electron microscopy and western blotting are use to detect the activtity of autophagy in the liver and lung tissue of newborn naked mole rat and C57BL/6. Reagents interfere the autophagy level of fibroblasts derived from naked mole rat and mouse. The cell number is tested after 24, 48, 72 hours by CCK-8 kit. Meanwhile, expression of Beclin 1 at each time point is detected by western blotting. Results Compared with mice, the naked mole rat tissue have significantly higher LC3B expression. The NMR cells have a certain amount of resistance from 3-MA. Rapamycin’s proliferation inhibition on NMR-S(fibroblasts derived from skin of naked mole rats)was significantly greater than that of mouse cells.Conclution NMR tissue possess a high autophagy, Cells from NMR have greater ability of self-regulation under extraneous autophagy inhibition, compared to C57BL/6.更多还原