【摘要】 目的 比较白毛黑眼(WHBE)与日本大耳白(JW)兔肠道淋巴细胞免疫活性的差异。方法 分别选取离乳和2月龄雄性WHBE兔、JW兔各8只，采用percoll分离液和Ficoll分离液，分别测定其小肠上皮层淋巴细胞; MTT法检测刀豆蛋白A(ConA)、脂多糖(LPS)对兔小肠上皮层淋巴细胞增殖的影响。结果 成年WHBE兔小肠淋巴细胞受ConA、LPS刺激的增殖程度比成年JW兔的明显升高，具有显著性差异。结论 WHBE兔的肠道淋巴细胞较JW兔更易受丝裂原的刺激作用而诱导激活，这可能是其肠易激的原因之一。更多还原

【Abstract】 Objective To investigate differences on immunological function of small bowel between white hair black eye(WHBE) rabbit and Japanese white(JW) rabbit. Methods The weanling and 2months old male WHBE rabbits and JW rabbits were used with 8 in each group. Two kinds of cell separation mediumsL?ficoll and percoll,were adopted for the isolation and purification. MTT test was applied to detect the lymphocyte proliferation induced by ConA and LPS. Results The levels of lymphocyte proliferation in small bowel of grown WHBE rabbits were much higher than those of grown JW rabbit. Conclusions Immunological function of lymphocytes in small bowel of grown WHBE Rabbit was much weaker than that of Japanese Rabbits. It may warrant further evaluation as a possible animal for the research of irritable bowel syndrome.更多还原