【摘要】 目的 复制乙酸性大鼠胃溃疡模型，用奥美拉唑治愈后继以白介素-1β诱导复发，建立溃疡复发的新模型。方法 1)采用改良Okabe乙酸涂抹法制作大鼠胃溃疡模型。2)溃疡大鼠，随机分为自然愈合组和治疗组，分别灌胃生理盐水和奥美拉唑。治疗组于第14、第21和第28日随机抽样，与自然愈合28 d大鼠一并处死，计算溃疡愈合率和溃疡指数。3)奥美拉唑治愈的溃疡大鼠，随机分为2组，分别腹腔注射IL-1β 0.75 mg/kg和IL-1β1.00 mg/kg,48 h后处死，评定溃疡复发率和溃疡指数。4)HE染色观察愈合和复发大鼠胃黏膜组织病理学改变。结果 1)抽样观察乙酸涂抹的大鼠，均表现为典型的胃溃疡。2)奥美拉唑治疗14 d、21 d和28 d后，溃疡愈合率分别为25.0%、37.50%和100.0%。愈合的溃疡组织:再生黏膜厚度随着治疗时间的增加而变厚;囊状扩张腺体数量、炎症细胞数量和微血管数量则随着治疗时间的增加而减少。自然愈合组在28 d的溃疡愈合率为37.50%。3)IL-1β(1.00μg/kg)组复发率为100%，溃疡指数为18.0±3.4;与奥美拉唑治疗28 d后愈合的溃疡组织相比，再生黏膜厚度明显变薄，囊状扩张腺体数量、炎症细胞数量和微血管数量则明显增加。结论 建立了乙酸性胃溃疡经奥美拉唑治愈后白介素-1β诱导复发的大鼠模型，适用于消化性溃疡临床治愈后复发的防治研究。更多还原

【Abstract】 Objective To establish the IL-1β-induced recurrence model in rat with acetic acid-induced gastric ulcer and cured by Omeprazole. Methods 1) The acetic acid-induced gastric ulcer rat model was reproduced by topical application of acetic acid, an improved method of Okabe. 2) Then, the rats with gastric ulcer were divided into 2 groups randomly, the rats of control group(spontaneous closure group) were intragastric administrated with physiological saline, while the rats of therapy group were administrated with Omeprazole. The rats Randomly sampled on the 14th, 21th and 28th day after treatment,together with the rats on the 28th day in control group, were executed at one time. Then the care rates(%), ulcer index, and the histopathological changes of gastric mucosa were assessed. 3) The cured rats were randomly divided into 2 groups, and were intraperitoneally injected with IL-1β of 0.75 mg/kg and1.00 mg/kg respectively. The recurrence rate of ulcer(%), ulcer index, and histopathological changes of gastric mucosa were assessed 48h later. Results 1) The model rats induced by acetic acid showed typical ulcer when dissected randomly for observation. 2) After 14, 21 and 28 days’ administration of Omeprazole, the cure rates were 25.0%, 37.50% and 100.0%, respectively. The thickness of regenerative gastric mucosa increased with the extension of therapy time. While the number of cystic-dilated glands,inflammatory cells and capillary vessels reduced as the treatment time increased. The cure rate of spontaneous closure with 28 days was 37.50%. 3) In the IL-1β-1.00 mg/kg group, the recurrence rate of ulcer was 100%, and the ulcer index was 18.0 ± 3.4. Moreover, compared with rats 28 days after treatment,the regenerative gastric mucosa was significantly thinned, but the number of cystic-dilated glands, inflammatory cells and capillary vessels obviously increased. Conclusion The model of interleukin1β-induced recrudescence model in rat with acetic acid-induced gastric ulcer which has been treated by Omeprazole was applicable to the study of prevention and treatment of recurrence of peptic ulcer.更多还原