【摘要】 探讨银杏叶提取物(extract of Ginkgo biloba,EGB)对于双酚A(Bisphenol A,BPA)活化RBL-2H3(简称:RBL)表达IL-4和TNF-α的影响。利用BPA激活RBL,通过测定β-氨基已糖苷酶和RT-PCR,研究EGB对于活化RBL脱颗粒以及表达IL-4和TNF-α的影响。结果显示,经BPA活化的RBL,加入EGB(浓度为160μg/ml)后,其β-氨基已糖苷酶释放仅为对照的45%,其IL-4mRNA表达量降低到对照的33.5%,TNF-αmRNA表达量降低到对照的26.8%。结果说明EGB极大地抑制了活化RBL的脱颗粒,同时对于活化RBL表达IL-4和TNF-α也具有极大的抑制作用,提示EGB治疗哮喘的作用与其抑制肥大细胞表达IL-4和TNF-α有关。更多还原

【Abstract】 To investigate the effect of Ginkgo biloba extract(EGB) on expression of IL-4 and TNF-α in BPA(Bisphenol A)-treated basophilic cell line RBL-2H3(RBL) cells,RBL was stimulated with BPA,and the β-hexosaminidase secretion was detected.The expression of IL-4 and TNF-α in RBL cells was measured by RT-PCR.At the dosage of 160μg/ml of EGB,the value of β-hexosaminidase activity in BPA-treated RBL decreased by 55%,and the IL-4 and TNF-α mRNA expression levels decreased to 33.5% and 26.8% respectively.Our results indicated that EGB inhibited the release of β-hexosaminidase and the expression of IL-4 and TNF-α in BPA-treated RBL-2H3 cells,which might be the mechanism of EGB in the treatment of asthma.更多还原