【摘要】 目的:探讨子宫内膜异位症(EMT)患者腹腔液中瘦素及白细胞介素-8(IL-8)表达及与其临床特征的相关性,探讨二者在EMT发病中的作用。方法:收集46例EMT患者(EMT组)及31例非EMT患者(对照组)的腹腔液,采用酶联免疫吸附试验(ELISA)法定量检测两组腹腔液中瘦素及IL-8的表达水平,分析瘦素、IL-8与EMT患者一般情况、分期及痛经或慢性盆腔痛评分的相关性。结果:腹腔液中瘦素、IL-8的表达水平与EMT患者年龄、体重、身高、体重指数及孕次均无相关性(r=-0.0900~0.1400,P均>0.05)。EMT组腹腔液中瘦素、IL-8表达水平(5.8±1.7ng/ml、75.0±23.3 pg/ml)分别高于对照组(2.2±0.9 ng/ml、18.6±9.4 pg/ml)(P<0.01)。瘦素在早期EMT组腹腔液中的表达水平(7.2±2.1 ng/ml)高于晚期EMT组(3.9±1.6 ng/ml)(P<0.01),而晚期EMT组的IL-8表达水平(138.0±31.4 pg/ml)高于早期EMT组(59.6±13.2 pg/ml)(P<0.05)。瘦素表达水平与rFAS分期呈负相关(r=-0.5615,P<0.05),IL-8表达水平与rFAS分期呈正相关(r=0.6320,P<0.01),晚期EMT患者IL-8表达水平与痛经或慢性盆腔痛评分呈正相关(r=0.6104,P<0.05)。盆腔EMT组的瘦素表达水平(9.7±2.0 ng/ml)高于卵巢巧克力囊肿组(4.3±1.6 ng/ml)(P<0.05)。结论:EMT患者腹腔液中瘦素及IL-8异常增高,升高的瘦素和IL-8可能起着促进、维持EMT病变的作用。瘦素可能与EMT的早期发病有关,而IL-8可能与EMT的进展有关。更多还原

【Abstract】 Objective:To compare levels of leptin and IL-8 in peritoneal fluid in women with endometriosis,and to explore the two angiogenic cytokines for a possible role in the pathophysiology of endometriosis.Methods:Peritoneal fluid from 46 women with endometriosis and 31women undergoing hysteromyoma ectomy was collected at laparoscopy.Leptin and IL-8 levels were detected using ELISA.The correlation of letin and IL-8 in stage,rFAS,type and presentation of endometriosis was analyzed.Results:Presence of age,weight,height,BMI and gravidity did not show any significant correlation with both leptin and IL-8(r =-0.0900 ~ 0.1400,P 均>0.05).Significantly higher leptin and IL-8 were demonstrated in peritoneal fluid in patients with endometriosis comparing with control(P<0.01).Levels of leptin were significantly higher in early stage than advanced stage(P<0.05),contrary to leptin,IL-8 levels were significantly elevated in those with advanced disease(P< 0.05).Levels of leptin correlated negatively with rFAS(r =-0.5615,P<0.05),and levels of IL-8 correlated positively with rFAS(r =0.6320,P<0.01).Pain scores were positively correlated with levels of IL-8 in women with advanced endometriosis(r = 0.6104,P<0.05).Women with peritoneal implants had significantly higher IL-8 levels than those with endometriotic cysts.Conclusions:Leptin and IL-8 levels are raised in peritoneal fluid of women with endometriosis,which reflects inflammation and dysregulated immunomodulation.Higher levels of leptin were correlated with early stages,and IL-8 seems to stimulate endometriosis aggravation in a dose-dependent manner.更多还原