【摘要】 目的探讨脑外伤时不同时期脑脊液IL-1β、IL-6、IL-8、TNF-α、IL-10的水平变化及临床意义。方法选择48例脑外伤患者,其中重型26例、轻型22例;高颅内压25例、低颅内压23例。分别留取伤后12、24、36、72 h的脑脊液标本,采用酶联免疫吸附法检测伤后各时间点脑脊液IL-1β、IL-6、IL-8、TNF-α、IL-10水平。另取查体健康者28例为对照组。结果与对照组相比,脑外伤患者脑脊液中IL-6、IL-1β、TNF-α、IL-8和IL-10水平均显著增高(P均<0.01)。脑外伤重型组脑脊液IL-6、IL-1β、TNF-α和IL-8峰值水平高于与轻型组(P均<0.01);高颅内压组脑脊液中IL-1β峰值水平高于低颅内压组(P<0.01)。结论脑外伤后脑脊液炎性细胞因子水平升高,可作为预测脑损伤严重程度的指标。更多还原

【Abstract】 Objective To investigate the concentration change of the inflammatory cytokines(IL-1β,IL-6,IL-8,TNF-α,IL-10) in cerebrospinal fluid at different periods of time after traumatic brain injury.Methods In this study,48 patients with traumatic brain injury were enrolled,the cerebrospinal fluid samples were collected in 12 h,24 h,36 h and 72 h after traumatic brain injury,the concentration of the inflammatory cytokines were detected with enzyme-linked immunosorbent assay.Results Compared with that in control group,there were significantly increased concentrations of IL-1β,IL-6,IL-8,TNF-α,IL-10 in patients with traumatic brain injury(all P<0.01).Peak concentrations of IL-6,IL-1β,TNF-α and IL-8 of cerebrospinal fluid in patients with severe brain injury were significantly higher than those in patients with mild brain injury(all P<0.01).Peak concentration of IL-1β in patients with high intracranial pressure was higher than that in patients with low intracranial pressure(P<0.01).Conclusion The levels of IL-1β,IL-6,IL-8,TNF-α and IL-10 increase after the traumatic brain injury,which may be one of the probable biomarkers for the severity of brain injury.更多还原