【摘要】 目的:研究钩藤碱、异钩藤碱和钩藤总生物碱对自发性高血压大鼠(SHR)肠系膜动脉平滑肌细胞凋亡和增殖的影响。方法:将SHR分组,分别给予钩藤碱、异钩藤碱和钩藤总生物碱,并以Wistar大鼠作为正常对照。采用HE染色法观测肠系膜动脉的病理形态,采用Masson染色法观察肠系膜动脉胶原含量,采用免疫组化法检测肠系膜动脉Bcl-2、Bax、c-myc、c-fos、MMP-9和TIMP-2的蛋白表达。结果:钩藤碱、异钩藤碱和钩藤总生物碱能减轻SHR肠系膜动脉的病理损害,降低动脉壁的胶原沉积,下调肠系膜动脉平滑肌Bcl-2、c-fos和TIMP-2蛋白表达,钩藤碱上调肠系膜动脉MMP-9蛋白表达。结论:钩藤碱和异钩藤碱可能通过下调原癌基因Bcl-2蛋白表达,促进SHR肠系膜动脉细胞凋亡,通过下调c-fos蛋白表达途径抑制SHR肠系膜动脉细胞异常增殖,并通过调节MMP-9和TIMP-2蛋白表达抑制SHR肠系膜动脉的胶原沉积,从而改善肠系膜动脉壁重塑。更多还原

【Abstract】 Objective:To study the apoptosis and proliferation effect of rhynchophylline,isorhynchophylline and uncaria alkaloids on spontaneously hypertensive rat(SHR) mesenteric artery smooth muscle cells.Methods:SHR were divided into different groups,and given rhynchophylline,isorhynchophylline and uncaria alkaloids.Wistar rats were used as normal control.Mesenteric artery pathology was observed by HE staining,mesenteric artery collagen content by Masson staining.Bcl-2,Bax,c-myc,c-fos,MMP-9 and TIMP-2 protein expression of mesenteric artery were detected by immunohistochemical.Results:Rhynchophylline,isorhynchophylline and uncaria alkaloids could alleviate the pathological damage of the SHR mesenteric arteries,reduce arterial wall collagen deposition,and lower the protein expression of Bcl-2,c-fos and TIMP-2 of mesenteric artery smooth muscle.Rhynchophylline increased mesenteric artery MMP-9 protein expression.Conclusion:Rhynchophylline and isorhynchophylline may promote apoptosis of SHR mesenteric arteries by lowering the proto-oncogene Bcl-2 protein expression,inhibite mesenteric artery cells abnormal proliferation by down-regulating c-fos protein expression pathway,and inhibite collagen deposition through regulating MMP-9 and TIMP-2 protein expression.Thereby the mesenteric arterial walls were improved.更多还原