【摘要】 目的研究血管紧张素(1-7)(Ang(1-7))及培哚普利对实验性糖尿病大鼠视网膜上TGF-β1表达的影响,探讨Ang(1-7)及培哚普利对糖尿病视网膜病变的保护作用。方法选择健康的雄性8周龄Wistar大鼠为研究对象。采用一次性腹腔注射链脲佐菌素(STZ,65 mg/kg)的方法建立糖尿病大鼠模型。随机分为5组:a组为Ang(1-7)组(400 ng.kg-1.mim-1),b组为培哚普利组(2 mg.kg-1.d-1),c组为Ang(1-7)+培哚普利组[Ang(1-7),400 ng.kg-1.mim-1+培哚普利,2 mg.kg-1.d-1],d组为糖尿病对照组,e组为正常对照组。采用免疫组化的方法检测各组大鼠视网膜上TGF-β1蛋白的表达。结果 Ang(1-7)组、培哚普利组及糖尿病对照组TGF-β1的表达高于正常对照组(P均<0.01),Ang(1-7)+培哚普利组与正常对照组差异无统计学意义(P>0.05),Ang(1-7)组、培哚普利组、Ang(1-7)+培哚普利组TGF-β1的表达明显低于糖尿病对照组(P均<0.01),Ang(1-7)组、培哚普利组TGF-β1的表达高于Ang(1-7)+培哚普利组(P<0.01)。结论糖尿病视网膜中TGF-β1的表达高于正常对照组,Ang(1-7)和培哚普利能够有效抑制糖尿病大鼠视网膜TGF-β1的表达,Ang(1-7)与培哚普利联合应用能够更有效抑制糖尿病大鼠视网膜TGF-β1的表达,对糖尿病大鼠视网膜具有保护作用。更多还原

【Abstract】 Objective To study the protective function of Angiotensin(1-7)(Ang(1-7)) and Perindopril in diabetic retinopathy(DR) by assessing their effect on expression of TGF-β1 in eyes of experimental diabetic rats.Methods The healthy male 8-week-old Wistar rats were enrolled in this study.Diabetes was induced by intraperitoneal injection of streptozotocin(STZ,65 mg/kg).The rats were then randomly allocated into five groups: a.Ang(1-7) group,treated with Ang(1-7) at(400 ng·kg-1·min-1);b.Perindopril group,treated with Perindopril at(2 mg·kg-1·d-1);c.Ang(1-7)+perindopril group,treated with [Ang(1-7),400 ng·kg-1·min-1,and perindopril,2 mg·kg-1·d-1];d.Diabetes group,e.non-diabetic control group.Expression of TGF-β1 was assessed by immunohistochemical assay.Results Expression of TGF-β1 in the retina in the Ang(1-7) group,perindopril group and diabetes group was higher than that in the non-diabetic control group(P<0.01,P<0.01,P<0.01),while it in the Ang(1-7)+perindopril group was not significantly difference with that in the non-diabetic control group(P>0.05).Expression of TGF-β1 in the retina in the Ang(1-7) group,Perindopril group and Ang(1-7)+perindopril group was lower than that in the diabetes group(P<0.01,P<0.01,P<0.01)and it in the Ang(1-7) group and the Perindopril group was higher than that in the Ang(1-7)+perindopril group(P<0.01,P<0.01).Conclusion Ang(1-7) and perindopril can decrease the expression of TGF-β1 in the retina of rats with DR,and their combination can much effectively inhibit the expression of TGF-β1 and may prevent the development of DR.更多还原