【摘要】 以陕西省千阳县种羊场的足月正常分娩、健康的西农萨能羊为试验材料,运用PCR方法对其FASN基因启动子部分序列进行了克隆和序列分析,获得了西农萨能羊FASN基因启动子序列片段719 bp(GenBank收录号EF556550.1)。序列分析显示,TATA盒位于-35 bp处,1个类似于CAAT盒的序列位于-68 bp处,这些都是典型的真核生物启动子元件,TATA盒上游区域有76%以上的G/C含量和4个GC盒(GGGCGG和CCGCCC),潜在的核转录因子结合位点Sp1位于-99 bp、-174 bp、-255 bp和-320 bp。更多还原

【Abstract】 Using normal delivery and healthy Xinong Saanen goat from Qianyang Goat Seed Farm of Shanxi Province as experimental materials,the partial sequence of FASN gene promoter region was cloned and analyzed in the present work. Fragment length of FASN gene promoter cloned from Xinong Saanen goat was 719bp( GenBank accession no. EF556550. 1). TATA box was located in- 35 bp,and a similar CAAT box was located in- 68 bp,both of which were the typical eukaryotic promoter elements. The upstream domain of TATA box contained 76% G / C and four GC boxes( GGGCGG and CCGCCC),and potential binding sites of nuclear transcription factor Sp1 was at positions- 99 bp,- 174 bp,- 255 bp and- 320 bp.更多还原