【摘要】 建立了HPLC法测定忍冬的花、叶中木犀草苷和绿原酸含量的方法。应用Shim-pack ODS色谱柱(4.6×250 mm,5μm),乙腈/0.2%甲酸水梯度洗脱,流速为1.0 mL/min,检测波长为350 nm。在此色谱条件下,各组分在30 min内均得到良好分离。用该方法测定了3个品种忍冬的花、叶中木犀草苷和绿原酸的含量,结果表明亚特、意大利和九丰一号忍冬叶中木犀草苷含量分别为5.856、2.454和4.633 mg·g-1,明显高于花。更多还原

【Abstract】 We constructed a determination approach for the contents of luteoloside and chlorogenic acid in flowers and leaves from Lonicera japonica Thunb.with HPLC.Their compounds were completely separated under the conditions of Shim-pack ODS chromatographic column, gradient elution of Acetionitrile and 0.2% methanoic acid, flow rate of1.0 mL/min and detection wavelength of 350 nm.Results show that luteoloside content in the leaves of Yate,Italy and Jiufengyihao is 5.856,2.454 and 4.633 mg·g-1,much higher than that in their flowers.更多还原