【摘要】 采用单因素试验和响应曲面法对酸奶中嗜热链球菌H1的最适生长条件进行了研究和优化.根据回归分析得到的最适培养条件为:温度41.02℃,pH5.74,接种量0.31mL·50mL-1,盐浓度0.23%,此时菌株的光密度OD600为1.176.经实验验证,在该条件下菌株光密度OD600为1.162,其相对误差小于2%.更多还原

【Abstract】 The growth conditions of S.thermophilus H1were studied by single factor experiment and response surface methodology.The results showed that the optimal parameters for the growth conditions as follows:the temperature was 41.02℃,the pH was value 5.74,the inoculum size was 0.31mL·50mL-1,the salt concentration was 0.23%,and the OD600of strain HI was 1. 176.Under the optimal conditions the OD600of strain HI was 1.162,The relative error was bellow 2%.更多还原