【摘要】 建立了同时测定水产品中氧氟沙星、诺氟沙星、环丙沙星、恩诺沙星4种氟喹诺酮类药物残留量的高效液相色谱方法,并对提取和净化等前处理条件进行了优化。目标化合物采用高效液相色谱-荧光检测器检测,外标法定量。对草鱼、鳙鱼、大黄鱼、虾进行检测分析,添加4、10、100、1000μg/kg浓度水平时,回收率为68.5%~106.6%,相对标准偏差0.80%~9.16%,方法的检出限为1.5μg/kg,定量限为4μg/kg。更多还原

【Abstract】 A high performance liquid chromatographic(HPLC)method was developed for the simultaneous determination of 4 fluoroquinolone residues(enrofloxacin, ciprofloxacin, norfloxacin, ofloxacin) in aquatic product. The sample pretreatment such as extration and cleanup were optimized. Target compounds were detected by HPLC with a flurorescence detector, and quantified with external standard method. Detection and analysis on grass carp, bighead carp, Yellow croaker and shrimp samples indicated that the recoveries of 4 FQs were 68.5%~106.6% at four spiked levels of 4,10,100,1 000 μg/kg,and the RSDs were below 9.16%. The limit of detection(LOD)was 1.5 μg/kg,the limit of quanification (LOQ)was 4 μg/kg.更多还原