【摘要】 建立同时测定西洋参中7种人参皂苷含量的快速检测方法。方法:采用超高效液相色谱法,ACQUITY UPLC BEH C18色谱柱(2.1×50mm,1.7μm),以乙腈-水为流动相,梯度洗脱,流速:0.5ml/min,柱温35℃,进样体积2μL,检测波长203 nm。结果:人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rb3、Rd存在良好线性关系,线性范围分别为0.0067~0.334mg/ml,0.0151~0.756 mg/ml,0.0130~0.649 mg/ml,0.0043~0.217 mg/ml,0.0044~0.221mg/ml,0.0085~0.424 mg/ml,0.0086~0.43 mg/ml。结论:该方法准确、重现性好,可用于西洋参中人参皂苷含量测定。更多还原

【Abstract】 To develop the method for the content determination of seven ginsenoside in Panax ginseng. Method:This assay was performed on ACQUITY BEH C18(2.1 mm × 50 mm,1.7 μm) column with acetonitrile-water as mobile phase in gradient elution at a flow rate of 0.5mL min-1.The column temperature was 35℃.The injection volume was 2μL.And the detection wavelength was 203 nm. Result:There was a good linear relationship among ginsenosides Rg1、Re、Rb1、Rc、Rb2、Rb3、Rd in 0.0067 ~0.334mg/ml,0.0151 ~0.756 mg/ml,0.0130 ~0.649 mg/ml,0.0043 ~0.217 mg/ml,0.0044 ~ 0.221mg/ml,0.0085 ~0.424 mg/ml,0.0086 ~0.43 mg/ml respectively. Conclusion:this method is accurate and good reproducibility suitable for the measurement of ginsenosides in the roots of Panax quinguefolium L.更多还原