【摘要】 目的:通过观察参地颗粒对抗肾小球基底膜肾炎大鼠血、尿生化指标、肾脏组织病理以及肝细胞生长因子(HGF)和转化生长因子-β1(TGF-β1)在肾组织表达的影响,探讨其对大鼠抗肾小球基底膜肾炎的干预作用。方法:建立大鼠抗肾小球基底膜肾炎模型,随机分为病理对照组、参地颗粒组、肾炎舒组及正常对照组。监测实验第4天及第4、8周24h尿蛋白定量、血生化指标动态变化,观察肾组织病理形态学变化,应用免疫组化法观察大鼠肾组织HGF、TGF-β1的表达。结果:造模后第4天开始,病理对照组、肾炎舒组、参地颗粒组的24h尿蛋白定量、血生化指标较正常对照组比较有显著升高(P<0.05),之后一直维持较高水平;治疗8周后,参地颗粒组的24h尿蛋白定量、血肌酐和尿素氮水平与同期病理对照组、肾炎舒组比较,差异均有显著性意义(P<0.05)。免疫组化显示,病理对照组大鼠的HGF、TGF-β1蛋白表达半定量积分与正常对照组相比有显著性上升(P<0.05);而参地颗粒组和肾炎舒组的HGF半定量积分高于病理对照组(P<0.05),TGF-β1半定量积分低于病理对照组(P<0.05)。结论:参地颗粒对抗肾小球基底膜肾炎大鼠具有较好的肾脏保护作用。其作用机制可能是通过促进肾脏HGF分泌,抑制TGF-β1产生;同时也进一步增强HGF对TGF-β1的抑制作用来发挥对肾脏的保护作用。更多还原

【Abstract】 Objective:To investigate the influence of Shendi Granules(SG) on expressions of hepatocyte growth factor(HGF)and transforming growth factor-β1(TGF-β1)as well as blood and urine biochemical indicators in rats with anti-glomerular basement membrane nephritis(anti-GBM nephritis),and to explore the intervention effect on anti-GBM nephritis.Methods:The rats model of anti-GBM nephritis was established,and then were randomly divided into 4 groups,and they were model group,SG group,Shenyan Shu group and normal group.Urinary protein and blood parameters were detected after 4 days,4 weeks,8 weeks respectively.The renal pathological changes were observed after HE,PAS and Masson staining.Immunohistochemical assay was adopted to observe HGF and TGF-β1 protein expression in the renal tissue.Results:From day 4 after the modeling,the 24-hour urinary protein(24hUP),serum creatinine(SCr)and blood urea nitrogen(BUN)were significantly higher in model group,SG group and Shenyan Shu group than those in normal group(P<0.05),and remained high level from then on.After treatment for 8 weeks,24hUP,SCr and BUN levels were lower in SG group than those in the model group and Shenyan Shu group(P<0.05).The results of immunohistochemical assay showed that the model group had higher semi-quantitation scores of HGF and TGF-β1 protein expression than the normal group(P<0.05),and SG group and Shenyan Shu group had higher HGF expression level while lower TGF-β1 than the model group(P<0.05).Conclusion:Shendi Granules shows certain renal protective effect on anti-GBM nephritis rats.The mechanism may be relate to elevating the expression of HGF and reducing the production of TGF-β1,simultaneously,Shendi Granules promotes the inhibitive effect of HGF on TGF-β1.更多还原