Crude glucan from Pleurotus eryngii cell wall was subjected to CMC ion exchange chromatography and Sephadex G-75 gel column chromatography. Then a chromatographically pure gulcan(PEWP) was successfully isolated. The purity,molecular weight and structure of PEWP were measured through the high performance liquid chromatography(HPLC),infrared spectroscopy(IR) and chemistry analysis in the preliminary study. The results showed that the molecular weight of PEWP was 41 209 u and the monosaccharide unit was identi...