【摘要】 目的研究毛萼乙素抑制肝癌肿瘤细胞增殖的机制。方法利用MTT实验检测毛萼乙素对肝癌肿瘤细胞株增殖的影响,采用Hoechst染色法检测毛萼乙素是否能引起人肝癌细胞株HepG2的凋亡,采用Western-blotting检测凋亡相关蛋白Bax和PARP的表达水平,免疫荧光技术检测Bax的表达水平。结果毛萼乙素可以明显抑制肝癌细胞的生长,Hoechst染色实验发现毛萼乙素可以诱导肝癌细胞的凋亡,Western-blotting实验检测到实验组Bax高表达,凋亡蛋白PARP的剪切带与对照组相比也明显增强。免疫荧光实验也显示,实验组Bax高表达。结论毛萼乙素可通过升高促凋亡蛋白Bax的表达,促进肝癌细胞株HepG2的凋亡,从而抑制其增殖。更多还原

【Abstract】 Objective To explore the mechanism of the growth inhibitory effect of Eriocalyxin B (EriB) on HCC. Methods The growth inhibitory effect of EriB on HCC cells was measured by MTT assay. The effect of EriB on cell apoptosis was investigated by Hoechst staining. Apoptosis proteins were detected by Western-blotting and immunofluorescent staining. Results EriB had significant growth inhibitory effect on HCC cells in a dose-dependent manner. Hoechst staining indicated that EriB could induce cell apoptosis in hepatocellular carcinoma. And after treatment by EriB ,the apoptosis protein Bax,cleaved-PARP was increased in the experimental group. Immunofluorescent staining also confirmed the expression level of Bax in EriB treatment group was higher than that in the control group. Conclusion EriB may inhibit HCC growth by elevating the expression of Bax and promoting the HepG2 cells apoptosis.更多还原