【摘要】 目的分析小柴胡汤剂作用于日本血吸虫感染小鼠后的血清多肽变化,评价其治疗效应。方法构建日本血吸虫感染小鼠模型及小柴胡汤剂治疗组,利用弱阳离子磁珠纯化血清多肽,应用时间飞行质谱(MALDI-TOF-MS)检测并结合ClinProTool数据处理软件进行分析。结果在Mr800~17 000区段,与感染组小鼠血清多肽指纹图谱比较,对照组小鼠与小柴胡治疗组血清中均检测到3个差异表达的多肽峰,即Mr 2 924、8210的表达呈极显著上调(P<0.01);Mr 11 690呈显著下调(P<0.05)。结论小柴胡汤对治疗日本血吸虫病具有潜在的应用价值,并为从蛋白水平上筛选治疗靶点提供理论依据。更多还原

【Abstract】 Objective To analyze the sera peptides from mice infected with Schistosoma japonicum,by Chaihu,and evaluate its effect.Methods The infected mice model and the treated group were estabhshed,peptides were purified from the sera by MB-WCX kit(Bruker Daltonics GmBH),and then analyzed by MALDI-TOF-MS and ClinProTool algorithm.Results Among the mass range from 800 to 17 000 Da,expression of 2 924 Da and 8 210 Da were up-regulated and 11 690 Da down-regulated in the healthy and treated group compared with the infected group.Conclusion Chinese medicine Chaihu has the potential value in the treatment of schistosomiasis which provides theoratic basis for screening treating targets in protein level.更多还原