【摘要】 目的:研究CD4+CD25highCD127low/-调节性T细胞(Treg)在胃癌患者外周血中的表达水平,探讨其在肿瘤发病机制及治疗中的作用。方法:采用流式细胞术检测法检测90例胃癌患者(35例为早中期胃癌,55例为晚期胃癌)和30名健康体检者外周血中CD4+CD25highCD127low/-Treg的表达水平。结果:90例胃癌患者外周血CD4+T细胞中CD4+CD25highCD127low/-Treg含量为(11.60±5.99)%,高于健康体检者(5.19±1.72)%,t=5.610,P=0.007;Ⅲ+Ⅳ期患者外周血CD4+T细胞中CD4+CD25high CD127low/-Treg含量为(12.55±6.59)%,明显高于Ⅰ+Ⅱ期患者(10.39±4.68)%(t=4.113,P=0.04)和健康体检者(5.19±1.72)%(t=5.923,P=0.001)。手术后胃癌患者CD4+CD25highCD127low/-Treg水平为(6.12±2.13)%,明显低于手术前(11.25±5.63)%,t=5.237,P=0.04。结论:胃癌患者外周血CD4+CD25highCD127low/-Treg数量增多且与病程分期相关,手术后Treg数量明显减少,Treg可能参与了胃癌的发生发展。更多还原

【Abstract】 OBJECTIVE:To research the level of CD4+CD25highCD127low/-regulatory T cells(Treg) in the patients with gastric cancer and investigate the role in pathogenesis and treatment of cancer.METHODS:The peripheral blood from 30 healthy subjects and 90 gastric cancer patients were used to detect the proportion of CD4+CD25highCD127low/-Treg with the multi-color detection and multi-parameter flow cytometry.RESULTS: Compared with healthy group[(5.19±1.72)%],the proportion of CD4+CD25highCD127low/-Treg in the peripheral blood of cancer patients[(11.60±5.99)%] showed a significant increase(t=5.610,P=0.007).With the progress of the disease,the proportion of CD4+CD25highCD127low/-Treg in patients of stage Ⅲ+Ⅳ[(12.55±6.59)% ] was significantly higher than that in patients of stageⅠ+Ⅱ[(10.39±4.68)%,t=4.113,P=0.04] and healthy individuals[(5.19±1.72)%,t=5.923,P=0.001)].The proportion of CD4+CD25highCD127low/-Treg was significantly lower in postoperative patients[(6.12±2.13)%] than that in preoperative patients[(11.25±5.63)%,t=5.237,P=0.04].CONCLUSIONS: The proportion of CD4+CD25highCD127low/-Treg in the peripheral blood of cancer patients is related to the tumor stage,and its number in postoperative patients showed a significant reduction.CD4+CD25highCD127low/-Treg may be involved in the development of gastric carcinogenesis and may become an important molecular target for detection of the immune status of patients with gastric cancer.更多还原