【摘要】 目的:探讨胃癌组织HIF-1α的表达与幽门螺杆菌(Hp)感染的相关性,以及两者与胃癌临床病理特征之间的关系。方法:采用快速尿素酶试验和组织学改良Giemsa染色法联合检测130例胃癌组织与70例慢性胃炎组织中Hp感染情况,采用免疫组织化学PV9000法,检测130例胃癌组织与70例慢性胃炎组织中HIF-1α蛋白的阳性表达情况。结果:胃癌组明确感染Hp的患者有100例,Hp阳性感染率为72.0%,明显高于胃炎组的46.0%,χ2=9.703,P<0.01;胃癌组HIF-1α蛋白阳性表达率为56.0%,明显高于胃炎组的14.0%,χ2=24.138,P<0.01;Hp(+)组HIF-1α蛋白的阳性表达率为63.9%,明显高于Hp(-)组的35.7%,χ2=6.495,P<0.05;胃癌组织中Hp感染与HIF-1α蛋白表达之间存在正相关,r=0.255,P<0.05;Hp感染与胃癌组织浸润深度及有无淋巴结转移有关,P值均<0.05,而与组织分化程度无关,P>0.05。HIF-1α蛋白的表达水平与胃癌组织浸润深度(P<0.05)及有无淋巴结转移(P<0.01)有关,而与组织分化程度无关,P>0.05。结论:Hp感染与HIF-1α蛋白表达可能在胃癌的发生中起协同作用,并与胃癌的浸润深度及淋巴结转移等密切相关。更多还原

【Abstract】 OBJECTIVE: To reveal the correlation between the expression of the HIF-1α in gastric carcinoma tissues and Hp infection,and analyze the clinical pathological features of gastric carcinoma.METHODS:Tissue samples were collected from 130 patients with gastric carcinoma and 70 patients with chronic gastritis,Hp was identified by rapid urease test and modified Giemsa-staining.Immunohistochemical method(PV9000) was used to detect the expression of HIF-1α.RESULTS:Hp positive infection rate of 100 cases of gastric cancer tissue(72.0%) was significantly higher than that of chronic gastritis(46.0%,χ2=9.703,P<0.01);HIF-1α protein positive expression rate of gastric cancer tissue(56.0%) was significantly higher than that of chronic gastritis(14.0%,χ2=24.138,P<0.01);HIF-1α protein positive expression rate of Hp positive group(63.9%) was significantly higher than that in Hp negative group(35.7%,χ2=6.495,P<0.05);Hp infection and the expression of HIF-1α protein in gastric carcinoma tissue was positively correlated(P<0.05,r=0.255).Hp infection was correlated with depth of infiltration range and lymph node metastasis(both P<0.05),but not related with cell differentiation grade(P>0.05).The expression of HIF-1α protein in gastric carcinoma tissue was correlated with depth of infiltration and lymph node metastasis(P<0.05P<0.01),but not related with cell differentiation grade(P>0.05).CONCLUSION:Hp infection and the HIF-1α protein expression may play a synergistic role in the incidence of gastric carcinoma;both are closely related with the occurrence,the depth of invasion and lymph node metastasis of gastric carcinoma.更多还原