【摘要】 目的观察单核细胞趋化蛋白-1(MCP-1)、转化生长因子β1(TGF-β1)及结缔组织生长因子(CTGF)在链脲佐菌素(STZ)诱发的2型糖尿病肾病大鼠肾组织中的表达。方法将35只SD雄性大鼠随机分为对照组14只和糖尿病肾病组21只。糖尿病肾病组给予高脂饮食,产生胰岛素抵抗后一次性腹腔注射STZ,35 mg/kg;对照组给予常规饲料,腹腔注射等量的柠檬酸-柠檬酸钠缓冲液。采用免疫组织化学法检测两组大鼠肾组织3种因子的表达。结果 MCP-1、TGF-β1、CTGF在STZ诱导的2型糖尿病肾病大鼠肾组织中(主要在肾小管)有较强的表达,糖尿病肾病组大鼠3种因子的平均吸光度分别为(0.29±0.04)、(0.20±0.02)、(0.20±0.02),对照组大鼠分别为(0.21±0.02)、(0.17±0.01)、(0.16±0.02),差异均有统计学意义(P<0.05)。结论 MCP-1、TGF-β1、CTGF在STZ诱导的2型糖尿病肾病大鼠肾组织中表达增强,参与了糖尿病肾病早期的发病。更多还原

【Abstract】 Objective To investigate the expression of monocyte chemotactic protein-1(MCP-1),transforming growth factor beta1(TGF-β1) and connective tissue growth factor(CTGF) in the renal tissues of rats with STZ induced type 2 diabetic nephropathy.Methods Thirty-five Sprague-Dawley male rats were randomly divided into control group(N group,14 cases) and diabetic nephropathy model group(DN group,21 cases).The DN group include rats with insulin resistance induced by diets,the rats were given a single intraperitoneal injection of streptozotocin of STZ.The rats in N group were given conventional feed,and were given a single intraperitoneal injection of the same amount of citric acid-sodium citrate buffer.Immunohistochemisty were performed to examine the expression levels of three factor in renal tissue.Results The expression levels of MCP-1,TGF-β1 and CTGF were strong in the renal tissues of rats with STZ induced type 2 diabetic nephropathy,the average absorbance of three factors in the DN group was(0.29 ± 0.04),(0.20 ± 0.02),(0.20 ± 0.02) respectively,the average absorbance in the N group was(0.21 ± 0.02),(0.17 ± 0.01),(0.16 ± 0.02) respectively,there were significant difference between DN group and N group of three factors(P < 0.05).Conclusion The expression of MCP-1,TGF-β1 and CTGF were obviously increased in the renal tissues of rats with STZ induced type 2 diabetic nephropathy,which are involved in the pathogenesis of early diabetic nephropathy.更多还原