【摘要】 目的:研究溃疡性结肠炎(UC)患者肠黏膜蛋白酶激活受体2(PAR-2)及环氧合酶2(COX-2)表达情况及在UC发病机制中的作用。方法:UC患者36例,对照组33例,均行内镜检查并活检肠黏膜组织,应用组织病理学及免疫组织化学分析组织中PAR-2及COX-2表达情况。结果:UC患者肠黏膜PAR-2和COX-2表达均高于对照组(P<0.05)。重度UC组PAR-2及COX-2表达高于中度、轻度及对照组(P<0.05);病理Ⅰ～Ⅳ级病变肠黏膜中PAR-2表达均高于对照组(P<0.05),Ⅲ～Ⅳ级高于Ⅰ～Ⅱ级(P<0.05)。病理Ⅲ～Ⅳ级COX-2表达明显高于Ⅰ～Ⅱ级及对照组(P<0.05,P<0.01)。UC患者正规治疗,进入缓解期后两者表达明显下降(P<0.01)。在肠黏膜组织中PAR-2表达与COX-2表达呈正相关(r=0.501,P<0.01);均与UC病理分级呈正相关(P<0.05)。结论:UC的发生发展与PAR-2及COX-2过度表达密切相关,两者共同促进炎症的发生发展。更多还原

【Abstract】 Objective: To investigate the expression of PAR-2 and COX-2 in intestinal mucosa of patients with ulcerative colitis,and the possible roles of PAR-2 and COX-2 in the pathogenesis of UC.Methods: Experimental colonic tissues were obtained from 36 patients with UC undergoing colonoscopy.Normal colon tissues were obtained from 33 healthy controls(UC patients with lesion mucosa,control group with normal mucosa).Histopathological examination and Immunohistochemistry were used to determine the expression of PAR-2 and COX-2.Result: Severe UC group PAR-2 and COX-2 expression was higher than moderate,mild and control group. Expression of PAR-2 in the mucosa of patients with inflammation grade I～IV was significantly higher than that of the healthy contro(l P<0.05).Grade III～IV exhibited significant higher expression than that of grade I～II(P<0.05).The patients with inflammation grade III～IV exhibited significant higher expression of COX-2 than that of grade I～II and the healthy control(P<0.05 and P<0.01), however the patients with inflammation grade I～II had no significant different expression of COX-2(P>0.05).The expression of PAR-2 and COX-2 in the colonic lesions mucosa of patients with UC was significant higher than that of the no lesions mucosa(P<0.01).The expression of PAR-2 and COX-2 was significant lower after regular systemic treatment(P<0.01).The expression of PAR-2 was positively correlated with the expression of COX-2(r=0.501,P<0.01),and also positively correlated with the pathological grade of UC(P<0.05).Conclusion: The occurrence of UC correlated with the increased expression of PAR-2 and COX-2.PAR-2 and COX-2 had a close relationship and could jointly promote the development of inflammation.更多还原