【摘要】 目的:观察人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUCMSCs)移植对缺血性脑卒中模型大鼠行为的影响。方法:制作大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)局灶性脑缺血大鼠模型,随机分为移植组和对照组,2 d后将体外培养的第3代hUCMSCs经尾静脉移植入移植组大鼠体内,对照组大鼠注入等量生理盐水。用Longa评分、转棒实验及Morris水迷宫等行为学检测方法,评价细胞移植后模型大鼠行为学改善情况。结果:(1)自细胞移植后第7天起,移植组Longa评分优于对照组(P<0.05);(2)细胞移植20 d后,移植组大鼠在转棒上停留时间与对照组相比明显延长(P<0.05);(3)细胞移植30 d后,移植组大鼠逃避潜伏期短于对照组(P<0.05)。结论:hUCMSCs可促进MCAO局灶性脑缺血模型大鼠神经功能的恢复。更多还原

【Abstract】 Objective: To observe the effect of human umbilical cord mesenchymal stem cells(hUCMSCs) transplantation on the functional behavior recovery of focal ischemic rat models.Methods: The rats were used to establish the middle cerebral artery occlusion(MCAO) model and divided randomly into control group and transplantation group.Two days later,the passage-3 hUCMSCs(3×106) were transplanted into the model animals via tail vein.The rats in control group were given the same dose of 0.9% saline.The functional behaviors were assessed by using Longa score,rotarod test and Morris water maze test.Results:(1)Since the 7th day after transplantation,Longa score of animals in transplantation group was markedly more than that in the control group(P<0.05).(2)At the 20th day after transplantation,the maintanence time of the transplantation group in rotarod test was significantly prolonged compared with the control group(P<0.05).(3)The Morris water amaze test showed that the escape latency of the transplantation group were shorter than that of the control group(P<0.05).Conclusion: hUCMSCs transplantation could promote neurological functional recovery of the MCAO model rats.更多还原