【摘要】 为了进一步分析本次猪暴发高发病率和高死亡率疫情的病因,本研究利用病毒分离与鉴定方法,将4份RT-PCR鉴定为单纯PRRSV阳性的组织病料,接种Marc-145细胞,经RT-PCR、IFA和电镜鉴定成功的分离到4株PRRSV,TCID50为10-6.5/0.1ml,命名为BJSY-1、BJPG、BJSD和BJBLZ株。本研究建立的方法为PRRS的诊断及防制提供了技术保障。更多还原

【Abstract】 In order to further analyze the reason of outbreak of high morbidity and mortality of porcine,In this study,we used 4 tissue samples which without inoculated PRRS vaccine and were positive by RT-PCR identifying inoculated to Marc-145 cells for Isolation and identification of the virus.By RT-PCR,IFA and electron microscopy analysis proved that all the 4 strains of virus were PRRSV,the TCID50 were 10-6.5/0.1mL,named BJSY-1,BJPG,BJSD and BJBLZ strains.The method established by the experiment provided the technical support for diagnosis and prevention of PRRS.更多还原