【摘要】 利用定点突变技术获得了杀线虫活性增强的cry6 Aa2(GenBank登录号为DQ257287)突变子,同时参照拟南芥密码子使用情况和植物基因组特点,全基因合成了去除稀有密码子和不稳定元件的突变子基因,并将修饰改造后的cry6 Aa2突变子基因转入拟南芥(Arabidopsis thaliana),筛选获得了表达Cry6 Aa2突变蛋白的植株.用南方根结线虫(Meoidogyne incognita)侵染转基因拟南芥,结果显示转基因植株具有一定抗根结线虫的作用.为植物寄生线虫的防治和转基因植物的构建提供了参考,同时为深入研究植物寄生线虫和转基因抗虫植物的相互作用关系奠定了基础.更多还原

【Abstract】 An enhanced nematicidal mutant by site-directed mutagenesis was obtained.In order to express the cry6 Aa2 mutant in Arabidopsis thaliana,the gene was synthesized and modified by removing some destabilizing sequences and codons not common based on the Arabidopsis genome prior to introduce into A.thalianavia Agrobacerium rhizogenes.The Cry6Aexpressing plants could decrease the number of galls caused by root-knot nematode Meoidogyne incognita.更多还原