【摘要】 目的:探讨膜辅助蛋白(membrane cofactor protein,MCP)与宫颈癌细胞免疫逃逸之间的关系,揭示MCP在宫颈癌发生、发展中的作用。方法:以宫颈癌细胞系ME180、宫颈癌组织和癌旁正常组织为材料,采用real-time PCR和Western blot检测MCP基因mRNA和蛋白质表达水平;利用小干扰RNA(small interference RNA,siRNA)沉默MCP基因表达后,四甲基偶氮唑盐(MTT)比色法检查宫颈癌细胞的增殖,以Transwell评估宫颈癌细胞的迁移能力。结果:MCP的表达在宫颈癌组织中明显高于其配对的癌旁正常组织,抑制MCP基因的表达能显著降低ME180细胞的增殖、迁移能力。结论:MCP在宫颈癌细胞中的高表达可能是肿瘤细胞免疫逃逸的重要机制,在宫颈癌的发生发展中发挥重要作用。更多还原

【Abstract】 Objective:To investigate the effect of membrane cofactor protein(MCP)on the survival of human cervical ovarian cancer cell.Methods:The expression of MCP mRNA and protein was explored by real-time PCR and Western blot,meanwhile,the effect of MCP on the migration and proliferation of ME180 cell was measured by Transwell and methyl thiazolyl tetrazolium respectively.The augment of human cervical ovarian cancer cells was detected by using MTT method,and the migration of such cells were evaluated by Transwell.Results:The results showed that the expression of MCP significantly increased in human cervical cancer tissue,which contributed to increasing the cells migration and augment the number of ME180 cells.Conclusion:The high expression of MCP in human cervical ovarian cancer cells might be the key machanism of the survival of such cells,and play a vital role in the promotion process.更多还原