【摘要】 纳米铂粒子(PtNPs)具有良好的生物相容性及高的催化性能,利用恒电位法将DNA生物分子电沉积在PtNPs修饰电极表面,得到一纳米结构的导电薄膜,极大地增大了电极的比表面积,结合Nafion的高选择性,制备了一种新型的Nafion/DNA/PtNPs复合膜修饰电极,研究了多巴胺(DA)在该修饰电极上的电化学行为,利用示差脉冲伏安法(DPV)对DA进行了定量分析.结果证明,该复合膜修饰电极大大提高了DA的电化学响应,在0.1 mol/L pH 7.0磷酸盐缓冲溶液(PBS)中,DA的示差脉冲伏安峰电流与其浓度在0.01～0.1μmol/L和0.1～6.0μmol/L两个范围内呈良好的线性关系,检出限可达3.3 nmol/L.此外,该修饰电极可以经受较高浓度抗坏血酸(AA)和尿酸(UA)的干扰,用于盐酸多巴胺注射液中DA含量的测定,结果满意.更多还原

【Abstract】 A new nanocomposite film modified electrode was preparaed by electrodepositing DNA at the platinum nanoparticles(PtNPs)surface and then coating Nafion on the composite film.This modified electrode(Nafion/DNA/PtNPs)was applied to detect dopamine(DA)by using differential pulse voltammetry(DPV).The result indicates that the Nafion/DNA/PtNPs nanocomposite film has electrocatalytic effect on the oxidation of DA and results in an obvious enhancement of the current response.In 0.1 mol/L pH 7.0 phosphate buffer solution(PBS),the DPV peak heights are linear with DA concentration in the range of 0.01-0.1 μmol/L and 0.1-6.0 μmol/L with the detection limit of 3.3 nmol/L(S/N=3).In addition,the interferences of uric acid(UA)and ascorbic acid(AA)can be effectively diminished.Moreover,the Nafion/DNA/PtNPs/GCE was applied to the DA detection in dopamine hydrochloride injection with satisfied results.更多还原