【摘要】 比较多不饱和脂肪酸(PUFA)各有效成分抑制肺腺癌干细胞增殖及迁移能力,筛选出最强组分,并探讨其量效关系.对肺腺癌细胞株A549及SPC-A-1进行无血清培养,待肿瘤干细胞(CSC)微球形成后,每日分别加入50μmol/L的α-亚麻酸(LA)、二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)、c9,t11-共轭亚油酸(c9,t11-CLA)和t10,c12-共轭亚油酸(t10,c12-CLA),连续3d.分别行干细胞微球形成观察、干细胞微球定量分析,筛选出最强组分.加入不同浓度最强组分进行侵袭试验.干细胞微球定量分析显示:除LA外,EPA、DHA、c9,t11-CLA和t10,c12-CLA都可不同程度地抑制A549及SPC-A-1干细胞微球的形成.在两种细胞株中,与EPA及DHA组相比,同等浓度CLA组平均干细胞微球个数及单个微球干细胞密度均显著下降,表明两种CLA为抑制作用最强组分.选取最强组分CLA行侵袭试验,c9,t11-CLA、t10,c12-CLA均能不同程度地抑制肺癌干细胞的迁移及浸润能力,随着浓度的增加,抑制作用增加,两者混合物的抑制作用最强.结果说明,PUFA组分EPA、DHA、c9,t11-CLA和t10,c12-CLA对A549及SPC-A-1干细胞微球都有不同程度的抑制作用,其中c9,t11-CLA和t10,c12-CLA为最强组分.c9,t11-CLA和t10,c12-CLA体外试验能够不同程度的抑制肺腺癌干细胞的浸润和迁移能力,并具有剂量依赖性.更多还原

【Abstract】 The purpose of the study is to compare the effect of each polyunsaturated fatty acids(PUFA)active ingredient inhibiting the proliferation and invasion capacity of lung adenocarcinoma stem cells,and to screen the strongest component out and explore its dose-effect relationship.A549 and SPC-A-1 were cultivated under serum-free condition,and after the formation of cancer stem cells(CSC)microspheroids,50 μmol/L LA,EPA,DHA,c9,t11-CLA and t10,c12-CLA were added respectively every day for three days.The most potent components were selected by observing the formation of stem cell microsphoids and quantitative analysis of stem cell microsphoids.The most potent components in different concentrations were added to carry out the tumour invasion assay.The quantitative analysis results of stem cell microspheroids showed that EPA,DHA,c9,t11-CLA and t10,c12-CLA can inhibit the formation of A549 and SPC-A-1 stem cell microspheroids in varying degrees except LA.In these two cell lines,the average number of stem cell microspheroids and the density of single microspheroid stem cell were significantly decreased in the groups with same concentration CLA,compared with EPA and DHA groups,which showed that two CLA were the components having strongest inhibiting effects.The strongest component,CLA,was selected to carry out the tumour invasion assay,and the results showed that:both c9,t11-CLA and t10,c12-CLA could inhibit the abilities of invasion of lung cancer stem cell in varying degrees.With increase of concentration,the effects of inhibition were improved correspondingly,and the mixture of two CLA components had strongest inhibiting effects.PUFA components EPA,DHA,c9,t11-CLA and t10,c12-CLA all had inhibitive effects on A549 and SPC-A-1 stem cell microspheroids in varying degrees,and c9,t11-CLA and t10,c12-CLA are strongest components.The in vitro test showed that c9,t11-CLA and t10,c12-CLA could inhibit the abilities of invasion of lung adenocarcinoma stem cells in varying degrees,have dose dependence.更多还原