【摘要】 目的建立定量检测α-synuclein的ELISA夹心法。方法采用抗人α-synuclein单抗包被酶标板,以兔抗人α-synuclein多抗为夹心抗体、HRP标记羊抗兔IgG为检测抗体、重组人α-synuclein为标准品,建立检测α-synuclein的间接ELISA法,并对36例正常对照、27例路易体痴呆患者脑脊液样本进行了检测。结果建立的夹心ELISA法检测α-synuclein的线性范围为1.56～200 ng/ml,批内、批间变异系数分别为为7.51%和13.7%,36例正常对照、27例路易体痴呆患者脑脊液α-synuclein的含量比较差异有统计学意义(P<0.05)。结论成功建立了一种检测α-synuclein的夹心ELISA法。更多还原

【Abstract】 To establish an ELISA method for the detection of α-synuclein,the anti-human α-synuclein monoclonal antibodies and rabbit anti-human α-synuclein polyclonal antibodies were used as coating antibody and sandwich antibodies,respectively.Recombinated human α-synuclein as standard substance and HRP labeled goat anti-rabbit IgG as detector antibody,thus a sandwich ELISA quantitative immunological detection method for αsynuclein was established.Then the established method was used to determine α-synuclein level in 36 healthy volunteers and 27 Dementia of Lewy bodies(DLB) patients.The linear range of the detection was from 1.56 ng/ml to 200 ng/ml,while the coefficients of variation of within assay and between assays were 7.51% and 13.7%,respectively.This method showed that the concentration of α-synuclein in the CSF of DLB patients was lower than that in healthy human.All the result indicated that a stable sandwich ELISA for the detection of human α synuclein was developed successfully,which would be helpful to further study the role of α-synuclein in neurodegenerative disease,and set a foundation for development a commercial assay kit.更多还原